Journal
TALANTA
Volume 77, Issue 5, Pages 1808-1813Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2008.10.025
Keywords
Escherichia coli; Staphylococcus aureus; Rapid detection; Fluorescence; CdSe quantum dots
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Funding
- Eleventh Five-Year Plan Key Project of Anhui China [06003015B]
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The aim of this paper was to demonstrate a fluorescence measurement method for rapid detection of two bacterial Count by using water-soluble quantum dots (QDs) as a fluorescence marker, and spectrofluorometer acted as detection apparatus, while Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were as detection target bacteria. Highly luminescent water-soluble CdSe QDs were first prepared by using thioglycolic acid (TGA) as a ligand, and were then covalently coupled with target bacteria. The bacterial cell images were obtained using fluorescence microscopy. Our results showed that CdSe QDs prepared in water phase were highly luminescent, stable, and successfully conjugated with E. coli and S. aureus. The fluorescence method could detect 10(2)-10(7) CFU/mL total count of E. coli and S. aureus in 1-2 h and the low detection limit is 10(2) CFU/mL. A linear relationship of the fluorescence peak intensity and log total count of E. coli and S. aureus have been established using the equation Y = 118.68X - 141.75 (r = 0.9907). (C) 2008 Elsevier B.V. All rights reserved
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