Journal
TALANTA
Volume 77, Issue 1, Pages 455-459Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2008.06.048
Keywords
Glucose biosensor; NiO hollow nanospheres; Glucose oxidase; Chitosan
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Funding
- National Key Basic Research Program of China [2007CB310500]
- Chinese Ministry of Education [705040]
- National Natural Science Foundation of China [90606009]
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NiO hollow nanospheres were synthesized by controlled precipitation of metal ions with urea using carbon microspheres as templates, which were for the first time adopted to construct a novel amperometric glucose biosensor. Glucose oxidase was immobilized on the surface of hollow nanospheres through chitosan-assisted cross-linking technique. Due to the high specific active sites and high electrocatalytic activity of NiO hollow nanospheres, the constructed glucose biosensors exhibited a high sensitivity of 3.43 mu A/mM. The low detection limit was estimated to be 47 mu M (S/N = 3), and the Michaelis-Menten constant was found to be 7.76 mM, indicating the high affinity of enzyme on NiO hollow nanospheres to glucose. These results show that the NiO hollow nanospheres are a promising material to construct enzyme biosensors. (C) 2008 Published by Elsevier B.V.
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