Journal
TALANTA
Volume 76, Issue 3, Pages 522-527Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2008.03.042
Keywords
hydrophilic interaction liquid; chromatography; retention equation; stationary phases; selectivity; nucleoside
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Hydrophilic interaction liquid chromatography (HILIC) is an effective technique for separating polar compounds. But its retention equation has not been studied systematically yet. in this study, an appropriate retention equation was established by using eight nucleosides as model analytes and by comparing four retention models on six different HILIC columns. As a result, retention equation In k = a + b x InCB + c x C-B could be quantitatively described the retention factors with good accuracy in HILIC mode. Based on this equation, the retention times of eight nucleosides under the conditions by other mobile phase can be predicted on each column. All of the predicted relative errors of retention times were smaller than 5%. The established retention model was also successfully applied to predict retention times of the real Traditional Chinese Medicine-Carthaus tinctorius L. sample. (C) 2008 Elsevier B.V. All rights reserved.
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