Journal
TALANTA
Volume 74, Issue 5, Pages 1414-1419Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2007.09.017
Keywords
titanium oxide nanotubes; hemoglobin; electrochemistry; hydrogen peroxide; biosensors
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Titanium oxide nanotubes (TiO2-NTs) synthesized by the hydrothermal method had been prepared as the co-immobilization matrix to incorporate hemoglobin (Hb) successfully. The nanostructures of TiO2-NTs were investigated by X-ray diffraction and high-resolution electron microscopy. The Hb immobilized in TiO2-NTs had a similar structure to the native of Hb and retained its near-native conformations as characterized by the UV-vis and FT-IR spectroscopy. A couple of quasi-reversible redox peaks with a formal potential of -0.34 V (vs. SCE) in 0.10 M pH 7.0 phosphate buffered saline (PBS) were observed. The amperometric response of the immobilized Hb linearly to H2O2 concentration ranged from 4 mu M to 64 mu M with a detection limit of 4.637 x 10(-6) M and the high stability of the immobilized Hb in TiO2-NTs constituted a promising platform for the development of biosensors. (c) 2007 Elsevier B.V. All rights reserved.
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