Journal
TALANTA
Volume 75, Issue 4, Pages 1075-1081Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2008.01.036
Keywords
aflatoxin B1; wheat; beta-cyclodextrin; enhanced spectrofluorimetry; second order advantage; parallel factor analysis
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Determination of aflatoxin B1 (AFB1) in wheat has been accomplished by enhanced spectrofluorimetry in combination with second-order standard addition method (EF-SOSAM). The adopted strategy combined the use of parallel factor analysis (PARAFAC) for extraction of the pure analyte signal and the standard addition method, for a determination in the presence of matrix effect caused by wheat matrix. The method is based on the enhanced fluorescence of AFB1 by beta-cyclodextrin in 10% (w/w) methanol-water solution. After sample treatment and without any extended cleanup steps and derivatization process, four standard additions were performed for each sample. A specific PARAFAC model was built from three-way arrays formed by excitation-emission spectra and 5 measurements (sample plus 4 additions). The scores related to AFB1 were used for a linear regression in the standard addition method. Two naturally contaminated wheat and spiked wheat samples containing AFB1 in the range 0-18 mu g kg(-1) were analyzed by EF-SOSAM and compared with HPLC results. EF-SOSAM analysis of spiked wheat samples gave a good correlation with spiked values (R-2 > 0.990). The limit of detection of method was 0.9. mu g kg(-1) for the determination of AFB1 in wheat samples. (c) 2008 Elsevier B.V. All rights reserved.
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