Journal
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
Volume 59, Issue 1, Pages 48-52Publisher
TAYLOR & FRANCIS INC
DOI: 10.3109/19396368.2012.727946
Keywords
embryo culture; human serum albumin product; recombinant human serum albumin; redox state
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Funding
- Grants-in-Aid for Scientific Research [22591157, 22590203] Funding Source: KAKEN
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Albumin has multiple physiological functions in embryo culture, such as a chelator of heavy metals, free radical scavenger, pH and osmotic regulator, a stabilizer, growth factor carrier, a surfactant, and a nutrient. However, the commercially available human serum albumin (HSA) products may not be completely safe since they could be contaminated with viruses and prions. Recombinant human serum albumin (rHSA) has been reported to be as efficient as commercial HSA for fertilization and embryo development. Despite the possible benefits of rHSA, it has not been widely used for embryo culture due to its high cost of production. Our objective was to analyze the redox state of different types of HSA products and rHSA to define oxidative status batch variations of HSA and rHSA and to evaluate the optimal concentration of rHSA for mouse embryo culture. The redox state of the HSA and rHSA used in embryo culture media was found to vary widely. Redox variations were found among different HSA batches as well as among rHSA batches. The highest blastocyst development and hatching rates were obtained with rHSA used at a concentration of 0.05 mg/mL. We showed that very low concentrations of rHSA were most favorable for advanced mouse embryo development in culture.
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