Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 42, Issue 3, Pages 275-283Publisher
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2018.08.004
Keywords
MLSA; ANI; isDNA-DNA hybridization; FAME; MALDI-TOF MS
Categories
Funding
- National Science Centre, Poland, project Opus 9 [2015/17/B/NZ9/01730]
- Ministry of Science and Higher Education Republic of Poland from Leading National Research Centre (KNOW) programme 2012-2017 Faculty of Pharmacy with Subfaculty of Laboratory Medicine [MNiSW-DS-6002-4693-23/WA/12]
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Four Gram-negative, rod-shaped pectinolytic bacterial strains designated as 2M, 9M, DPMP599 and DPMP600 were subjected to polyphasic analyses that revealed their distinctiveness from the other Pectobacterium species. Strains 2M and 9M were isolated from Calla lily bulbs cultivated in Central Poland. DPMP599 and DPMP600 strains were isolated from Calla lily leaves from plants grown in Serbia. Phylogenetic analyses based on nine housekeeping genes (gapA, gyrA, icdA, pgi, proA, recA, recN, rpoA, and rpoS), as well as phylogeny based on the 381 most conserved universal proteins confirmed that Pectobacterium zantedeschiae strains were distantly related to the other Pectobacterium, and indicated Pectobacterium atrosepticum, Pectobacterium betavasculorum, Pectobacterium parmentieri and Pectobacterium wasabiae as the closest relatives. Moreover, the analysis revealed that Pectobacterium zantedeschiae strains are not akin to Pectobacterium aroidearum strains, which were likewise isolated from Calla lily. The genome sequencing of the strains 2M, 9M and DPMP600 and their comparison with whole genome sequences of other Pectobacterium type strains confirmed their distinctiveness and separate species status within the genus based on parameters of in silico DNA-DNA hybridization and average nucleotide identity (ANI) values. The MALDI-TOF MS proteomic profile supported the proposition of delineation of the P. zantedeschiae and additionally confirmed the individuality of the studied strains. Based on of all of these data, it is proposed that the strains 2M, 9M, DPMP599, and DPMP600 isolated from Calla lily, previously assigned as P. atrosepticum should be reclassified as Pectobacterium zantedeschiae sp. nov. with the strain 9M(T) (PCM2893 = DSM105717 = IFB9009) as the type strain. (C) 2018 Elsevier GmbH. All rights reserved.
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