Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 36, Issue 4, Pages 218-223Publisher
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2013.03.001
Keywords
Bgradyrhizobium retamae; Retama; Phylogeny; Symbiovar
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Funding
- ERDF from Consejeria de Economia, Innovacion y Ciencia (Junta de Andalucia) [P09RNM4746]
- Junta de Castilla y Leon
- Junta de Castilla-La Mancha
- Comunidad de Madrid (Regional Spanish Governments)
- MINECO
- MCI (Central Spanish Government)
- Junta de Comunidades de Castilla La Mancha [POII10-0211-5015]
- JAE-Doc researcher contract from CSIC
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In this paper we analyze through a polyphasic approach several Bradyrhizobium strains isolated in Spain and Morocco from root nodules of Retama sphaerocarpa and Retama monosperma. All the strains have identical 16S rRNA genes and their closest relative species is Bradyrhizobium lablabi CCBAU 23086(T), with 99.41% identity with respect to the strain Ro19(T). Despite the closeness of the 16S rRNA genes, the housekeeping genes recA, atpD and glnll were divergent in Ro19(T) and B. lablabi CCBAU 23086(T), with identity values of 95.71%, 93.75% and 93.11%, respectively. These differences were congruent with DNA-DNA hybridization analysis that revealed an average of 35% relatedness between the novel species and B. lablabi CCBAU 23086(T). Also, differential phenotypic characteristics of the new species were found with respect to the already described species of Bradyrhizobium. Based on the genotypic and phenotypic data obtained in this study, we propose to classify the group of strains isolated from R. sphaeroccupa and R. monosperma as a novel species named Bradyrhizobium retamae sp. nov. (type strain Ro19(T) = LMG 27393(T) = CECT 82611). The analysis of symbiotic genes revealed that some of these strains constitute a new symbiovar within genus Bradyrhizobium for which we propose the name retamae, that mainly contains nodulating strains isolated from Retama species in different continents. (C) 2013 Elsevier GmbH. All rights reserved.
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