Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 34, Issue 7, Pages 508-512Publisher
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2011.03.006
Keywords
Nocardia; Nocardiaceae; 16S rRNA gene; gyrB gene; Rapid genus identification; South Africa
Categories
Funding
- National Research Foundation
- NRF [2073133]
- Harry Crossley Foundation
- Medical Research Council of South Africa
- University Research Committee (University of Cape Town)
Ask authors/readers for more resources
In this study two actinomycete strains were isolated in Cape Town (South Africa), one from a compost heap (strain 202GMO(T)) and the other from within the fynbos-rich area surrounded by the horseracing track at Kenilworth Racecourse (strain C2). Based on 16S rRNA gene sequence BLAST analysis, the strains were identified as members of the genus Nocardia. Phylogenetic analysis showed that the strains clustered together and are most closely related to Nocardia flavorosea NRRLB-16176(T), Nocardia testacea JCM 12235(T), Nocardia sienata IFM 10088(T) and Nocardia carnea DSM 43397(T). This association was also supported by gyrB based phylogenetic analysis. The results of DNA-DNA hybridization and physiological tests allowed genotypic and phenotypic differentiation of both strains 202GMO(T) and C2 from related species. However, their high DNA relatedness showed that they belong to the same species. Strain 202GMO(T) was selected as the type strain to represent this novel species, for which the name Nocardia rhamnosiphila is proposed (=DSM 45147(T) = NRRL B-24637(T)). (C) 2011 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available