Journal
HUMAN VACCINES & IMMUNOTHERAPEUTICS
Volume 11, Issue 6, Pages 1361-1367Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/21645515.2015.1008879
Keywords
abrin toxin; B chain; bioterrorism; immunogen; vaccine candidate; AT; abrin toxin; ATB; abrin toxin B chain; RT; ricin toxin; RTB; ricin toxin B chain; E; coli; Escherichia coli; LD50; 50% lethal dose; i; p; intraperitoneal or intraperitoneally; PBS; phosphate-buffered saline solution; SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel electrophoresis; ELISA; enzyme-linked immunosorbent assay; pAb; polyclonal antibody
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Abrin toxin (AT) consisting of an A chain and a B chain is a potential agent for bioterrorism and an effective vaccine against AT poisoning is urgently required. In this study, AT B chain (ATB) was successfully expressed in the Escherichia coli (E. coli) and assessed the protection capacity against AT intoxication. The recombinant ATB (rATB) subunit induces a good immune response after 4 immunizations. All BALB/c mice immunized intraperitoneally (i.p.) with the purified rATB protein survived after challenged with 5 x LD50 of AT. Transfusion of sera from immunized mice provided passive protection in naive mice. Furthermore, histological findings showed that immunization with rATB decreased the severity of toxin-related tissue damage. This work indicates that the rATB protein may be a promising vaccine candidate against human exposure to AT.
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