Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 33, Issue 1, Pages 25-34Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.syapm.2009.10.002
Keywords
Xylophagous insect; Cerambycid symbiosis; Yeast-like symbiont; Mycetome; Endocytobiont; Sodalis; Panfungal FISH
Categories
Ask authors/readers for more resources
Using fluorescence in situ hybridization (FISH) techniques and PCR-based rDNA sequencing, gut microflora in the larvae of bark- and wood-inhabiting cerambycid beetles (Rhagium inquisitor, Tetropium castaneum, Plagionotus arcuatus and Leptura rubra [Coleoptera: Cerambycidae]) was investigated. A total of 12 novel ascomycetous yeast strains were isolated from the gut content. Panfungal and strain-specific oligonucleotide probes identified two yeast strains as Candida rhagii and Candida shehatae, which were colonizing specialized organs (mycetomes) adhering to the gut of R. inquisitor and L. rubra larvae, respectively. Fragments containing these organisms were constantly being released from the mycetomes into the gut lumen. Whereas the mycetome symbiont of T castaneum could not be identified, all larvae of this species harbored an additional bacterial endocytobiont in their gut epithelium. This novel gammaproteobacterium belonged to the Sodalis clade of insect symbionts, which includes the secondary endosymbiont of tsetse flies (Sodalis glossinidius) and the Sitophilus oryzae primary endosymbiont (SOPE). Extracellular gut flora of the investigated cerambycid larvae was comprised of Alpha-, Beta-, and Gammaproteobactetia, Actinobacteria, Firmicutes, Verrucomicrobia and Acidobacteria. However, the individual composition among investigated larvae was highly variable and supposedly depended on individual host nutrition. (C) 2009 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available