Deinococcus misasensis and Deinococcus roseus, novel members of the genus Deinococcus, isolated from a radioactive site in Japan

Two gamma- and UV-radiation resistant, Gram-positive, red- or pink-pigmented, rod-shaped, strictly aerobic, oxidase- and catalase-positive bacterial strains, TDMA-25(T) and TDMA-uv51(T), were isolated from fresh water collected at Misasa, a radioactive site in Japan. Phylogenetic analysis based on 16S rRNA gene sequences placed both in a distinct lineage in the family Deinococcaceae, and the highest degrees of sequence similarity determined belonged to Deinococcus maricopensis LB-34(T) (88.8-89.3%), Deinococcus pimensis KR-235(T) (86.4-86.7%) and Deinococcus yavapaiensis KR-236(T) (86.1%). The DNA G+C content of the strains was 53-58mol%. The major respiratory quinone was MK-8. The predominant fatty acids were C-15:0 iso, C-16:0 iso, C-13:0 iso, C-17:0 iso, C-16:0, C-13:0 anteiso, C-15:0 and C-12:0 iso. The strains degraded gelatin, casein, starch and Tween 80. Unique physiological characteristics, differences in their fatty acid profiles, and genotypic and phylogenetic features, differentiated strains TDMA-25(T) and TDMA-uv51(T) from closely related Deinococcus species. Hence, the two strains are described as novel species of the genus Deinococcus. The names Deinococcus misasensis sp. nov. (type strain TDMA-25(T) = JCM 14369 = NBRC 102116 = CCUG 53610) and Deinococcus roseus sp. nov. (type strain TDMA-uv51(T) = JCM 14370 = NBRC 102117 = CCUG 53611) are proposed. (C) 2007 Elsevier GrnbH. All rights reserved.