Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 31, Issue 4, Pages 287-301Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.syapm.2008.04.004
Keywords
Xinjiang; Wild legumes; Rhizobial diversity; Phylogeny; MLSA
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Funding
- National Basic Research Program of China [2006CB100206]
- National Program for Basic S and T Platform Construction [2005DKA21201-10]
- National Natural Science Foundation of China [30670001, 30400001]
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A total of 111 rhizobial strains were isolated from wild legumes in Xinjiang, an isolated region of northwest China. Nine genomic species belonging to four genera of Rhizobium, Mesorhizobium, Ensifer, and Bradyrhizobium were defined among these strains based on the characterization of amplified 16S ribosomal DNA restriction analysis (ARDRA), restriction fragment length polymorphism (RFLP) analysis of 16S-23S rDNA intergenic spacers (IGS), 16S rRNA gene sequencing and multilocus sequence analysis (MLSA). Twenty-five nodC types corresponding to eight phylogenetic clades were divided by RFLP and sequence analysis of the PCR-amplified nodC gene. The acid-producing Rhizobium and Mesorhizobium species were predominant, which may be related to both the local environments and the hosts sampled. The present study also showed the limitation of using nod genes to estimate the host specificity of rhizobia. (C) 2008 Elsevier GmbH. All rights reserved.
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