Journal
G3-GENES GENOMES GENETICS
Volume 5, Issue 11, Pages 2257-2266Publisher
OXFORD UNIV PRESS INC
DOI: 10.1534/g3.115.021584
Keywords
fungal protein effectors; diagnostic genetic markers; plant disease resistance breeding; multiparent genetic mapping populations; high-density crop genotyping; MPP; Multiparent Advanced Generation Inter-Cross (MAGIC); multiparental populations
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Funding
- United Kingdom Biotechnology and Biological Sciences Research Council (BBSRC) [BB/E007260/1]
- Australian Grains Research and Development Corporation
- Erasmus studentship fund
- COST Action 'SUSTAIN'
- BBSRC [BB/E007201/1, BB/E007260/1, BB/N00518X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/N00518X/1, BB/E007260/1, BB/E007201/1] Funding Source: researchfish
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The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world's most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infection process. The recent discovery of pathogen effectors has revolutionized disease resistance breeding for necrotrophic diseases in crop species, allowing often complex genetic resistance mechanisms to be broken down into constituent parts. To date, three effectors have been identified in P. nodorum. Here we use the effector, SnTox1, to screen 642 progeny from an eight-parent multiparent advanced generation inter-cross (i.e., MAGIC) population, genotyped with a 90,000-feature single-nucleotide polymorphism array. The MAGIC founders showed a range of sensitivity to SnTox1, with transgressive segregation evident in the progeny. SnTox1 sensitivity showed high heritability, with quantitative trait locus analyses fine-mapping the Snn1 locus to the short arm of chromosome 1B. In addition, a previously undescribed SnTox1 sensitivity locus was identified on the long arm of chromosome 5A, termed here QSnn.niab-5A.1. The peak single-nucleotide polymorphism for the Snn1 locus was converted to the KASP genotyping platform, providing breeders and researchers a simple and cheap diagnostic marker for allelic state at Snn1.
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