Journal
SYMBIOSIS
Volume 59, Issue 3, Pages 165-171Publisher
SPRINGER
DOI: 10.1007/s13199-013-0228-0
Keywords
Culture; Lichens; Fungi
Categories
Funding
- Fulbright Scholar Program
- Spanish government
- Fundacion Caja Madrid
- Department of Biology at Duke University
- National Science Foundation Assembling the Tree of Life [DEB-0919455]
- Pacific Northwest National Laboratory foundational scientific focus area under the genomic sciences program of the Department of Energy Biological and Environmental Research Program [112442]
- Direct For Biological Sciences
- Division Of Environmental Biology [0919455] Funding Source: National Science Foundation
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In this study we describe the techniques used to culture 25 mycobionts spanning three classes and five orders of the leotiomyceta (Ascomycota). We find that five media, including potato-carrot, malt extract-yeast extract (MY), Bold's basal medium with nitrogen (NMBBM), oatmeal, and yeast extract with supplements (YES), are sufficient to induce ascospore germination of many lichenizing fungi and are also suitable for maintaining growth of the culture over the long term. Regular physical disruption of the cultures in liquid media is recommended to stimulate continued growth. Genomes of five of these lichen-forming fungal strains have been sequenced. The identity of each culture was confirmed by sequencing the nuclear ribosomal internal transcribed spacer (ITS) or the mitochondrial small subunit (mitSSU) from each strain. Additionally, the level of sequencing in terms of total number of genes sequenced for each taxon is provided. All fungal cultures have been deposited in public culture collections and, therefore, are available to the scientific community for conducting in vitro experiments.
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