Journal
SURGERY FOR OBESITY AND RELATED DISEASES
Volume 10, Issue 4, Pages 671-678Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.soard.2013.12.019
Keywords
DNA methylation; Blood; Gastric bypass; Very low calorie diet
Categories
Funding
- Strategic Diabetes Program at Karolinska Institutet
- European Research Council Ideas Program (ICEBERG) [ERC-2008-AdG23285]
- Swedish Research Council
- Swedish Diabetes Association
- Strategic Research Foundation
- Knut and Alice Wallenberg Foundation
- Novo Nordisk Foundation
- Stockholm County Council
- EMBO long-term fellowship
- Medical Research Council [1356015] Funding Source: researchfish
- Novo Nordisk Fonden [NNF12OC1016320, NNF14OC0010883, NNF13OC0005961] Funding Source: researchfish
Ask authors/readers for more resources
Background: Early benefits of Roux-en Y gastric bypass (RYGB) are partly mediated by the caloric restriction that patients undergo before and acutely after the procedure. Altered DNA methylation occurs in metabolic diseases including obesity, as well as in skeletal, muscle eight months after RYGB. The objective of this study was to test whether promoter methylation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1 A), pyruvate dehydrogenase kinase isozyme-4 (PDK4), transcription factor A (TFAM), interleulcin-1 beta (IL1 B), interleukin-6 (IL6) and tumor necrosis factor-alpha (TNF) is altered in blood after a very low calorie diet (VLCD) or RYGB. Methods: Obese nondiabetic patients (n = 18, body mass index [BMI] 42.3 +/- 4.9 kg/m(2)) underwent a 14-day VLCD followed by RYGB. Nonobese patients (n = 6, BMI 25.7 +/- 2.1 kg/m(2)) undergoing elective cholecystectomy served as controls. DNA methylation of selected promoter regions was measured in whole blood before and after VLCD. A subgroup of seven patients was studied 1-2 days and 12 +/- 3 months after RYGB. Promoter methylation was measured using methylated DNA capture and quantitative real-time polymerase chain reaction (PCR). Results: VLCD decreased promoter methylation of PPARGC1 A. Methylation of PPARGC1 A, TFAM, IL1 B, IL6, and TNF promoters was changed two days after RYGB. Similar changes were also seen on day one after cholecystectomy. Moreover, methylation increased in PDK4, IL1 B, IL6, and TNF promoters 12 months after RYGB. Conclusion: RYGB induced more profound epigenetic changes than VLCD in promoters of the tested genes in whole blood. Changes in DNA methylation may contribute to the improved overall metabolic health after RYGB. (C) 2014 American Society for Metabolic and Bariatric Surgery. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available