Journal
STRUCTURE
Volume 22, Issue 11, Pages 1665-1676Publisher
CELL PRESS
DOI: 10.1016/j.str.2014.08.017
Keywords
-
Funding
- Old Dominion University
- [NSF_Bio_1356621]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1356621] Funding Source: National Science Foundation
Ask authors/readers for more resources
Major secondary structure elements such as alpha helices and beta sheets can be computationally detected from cryoelectron microscopy (cryo-EM) density maps with medium resolutions of 5-10 angstrom. However, a critical piece of information for modeling atomic structures is missing, because there are no tools to detect b strands from cryo-EM maps at medium resolutions. We propose a method, StrandTwister, to detect the traces of beta strands through the analysis of twist, an intrinsic nature of a beta sheet. StrandTwister has been tested using 100 beta sheets simulated at 10 angstrom resolution and 39 beta sheets computationally detected from cryo-EM density maps at 4.4-7.4 angstrom resolutions. Although experimentally derived cryo-EM maps contain errors, StrandTwister's best detections over 39 cases were able to detect 81.87% of the beta strands, with an overall 1.66 angstrom two-way distance between the detected and observed beta traces. StrandTwister appears to detect the traces of beta strands on major beta sheets quite accurately, particularly at the central area of a beta sheet.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available