Journal
STRUCTURE
Volume 21, Issue 9, Pages 1500-1508Publisher
CELL PRESS
DOI: 10.1016/j.str.2013.08.006
Keywords
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Funding
- Human Frontier Science Program [RGY0079/2009-C]
- Arnold and Mabel Beckman foundation (BYI program)
- NIH [R01GM096089, U54RR022220]
- NSF CAREER [1150287]
- Leverhulme Trust [RPG-2012-519]
- BBSRC [BB/K01692X/1]
- MRC Centenary Award [G0600084]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1150287] Funding Source: National Science Foundation
- BBSRC [BB/K01692X/1] Funding Source: UKRI
- MRC [G0600084] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/K01692X/1] Funding Source: researchfish
- Medical Research Council [G0600084] Funding Source: researchfish
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A detailed description of macromolecular assemblies in multiple conformational states can be very valuable for understanding cellular processes. At present, structural determination of most assemblies in different biologically relevant conformations cannot be achieved by a single technique and thus requires an integrative approach that combines information from multiple sources. Different techniques require different computational methods to allow efficient and accurate data processing and analysis. Here, we summarize the latest advances and future challenges in computational methods that help the interpretation of data from two techniques-mass spectrometry and three-dimensional cryo-electron microscopy (with focus on alignment and classification of heterogeneous subtomograms from cryo-electron tomography). We evaluate how new developments in these two broad fields will lead to further integration with atomic structures to broaden our picture of the dynamic behavior of assemblies in their native environment.
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