Journal
STRUCTURE
Volume 19, Issue 8, Pages 1170-1181Publisher
CELL PRESS
DOI: 10.1016/j.str.2011.05.010
Keywords
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Funding
- NIH [GM080642]
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The cyanobacterial Oscillatory Agardhii agglutinin (OAA) is a recently discovered HIV-inactivating lectin that interacts with high-mannose sugars. Nuclear magnetic resonance (NMR) binding studies between OAA and alpha 3,alpha 6-mannopentaose (Man alpha(1-3)[Man alpha(1-3)[Man alpha(1-6)]Man alpha(1-6)]Man), the branched core unit of Man-9, revealed two binding sites at opposite ends of the protein, exhibiting essentially identical affinities. Atomic details of the specific protein-sugar contacts in the recognition loops of OAA were delineated in the high-resolution crystal structures of free and glycan-complexed protein. No major changes in the overall protein structure are induced by carbohydrate binding, with essentially identical apo- and sugar-bound conformations in binding site 1. A single peptide bond flip at W77-G78 is seen in binding site 2. Our combined NMR and crystallographic results provide structural insights into the mechanism by which OAA specifically recognizes the branched Man-9 core, distinctly different from the recognition of the Dl and D3 arms at the nonreducing end of high-mannose carbohydrates by other antiviral lectins.
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