Journal
CELL REPORTS
Volume 10, Issue 3, Pages 317-325Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2014.12.031
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Funding
- US-Israel Binational Science Foundation
- Administral Anslat Foundation
- Kekst
- Shapell
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Pancreatic islet beta cells are organized in rosette-like structures around blood vessels and exhibit an artery-to-vein orientation, but they do not display the typical epithelial polarity. It is unclear whether these cells present a functional asymmetry related to their spatial organization. Here, we identify murine b cell edges, the sites at which adjacent cell faces meet at a sharp angle, as surface microdomains of cell-cell adhesion and signaling. The edges are marked by enrichment of F-actin and E-cadherin and are aligned between neighboring cells. The edge organization is E-cadherin contact dependent and correlates with insulin secretion capacity. Edges display elevated levels of glucose transporters and SNAP25 and extend numerous F-actin-rich filopodia. A similar b cell edge organization was observed in human islets. When stimulated, b cell edges exhibit high calcium levels. In view of the functional importance of intra-islet communication, the spatial architecture of their edges may prove fundamental for coordinating physiological insulin secretion.
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