Journal
CELL REPORTS
Volume 13, Issue 9, Pages 1949-1964Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2015.10.056
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Funding
- Cancer Research UK (CRUK) Program Grant [C157/A15703]
- European Research Council Advanced Investigator grant [294440]
- CRUK China Fellowship [C157/A11682]
- CRUK PhD studentship
- CRUK Beatson core program
- AICR grant
- Cancer Research UK [15703] Funding Source: researchfish
- European Research Council (ERC) [294440] Funding Source: European Research Council (ERC)
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Genetic co-depletion of the actin-severing proteins ADF and CFL1 triggers catastrophic loss of adult homeostasis in multiple tissues. There is impaired cell-cell adhesion in skin keratinocytes with dysregulation of E-cadherin, hyperproliferation of differentiated cells, and ultimately apoptosis. Mechanistically, the primary consequence of depleting both ADF and CFL1 is uncontrolled accumulation of contractile actin stress fibers associated with enlarged focal adhesions at the plasma membrane, as well as reduced rates of membrane protrusions. This generates increased intracellular acto-myosin tension that promotes nuclear deformation and physical disruption of the nuclear lamina via the LINC complex that normally connects regulated actin filaments to the nuclear envelope. We therefore describe a pathway involving the actin-severing proteins ADF and CFL1 in regulating the dynamic turnover of contractile actin stress fibers, and this is vital to prevent the nucleus from being damaged by actin contractility, in turn preserving cell survival and tissue homeostasis.
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