Journal
STEROIDS
Volume 78, Issue 9, Pages 920-926Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.steroids.2013.05.001
Keywords
Testosterone; Dihydrotestosterone; Adipogenesis; Cell commitment
Funding
- UCLA Department of Obstetrics and Gynecology
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
- NIH [U54 HD071836]
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Androgens play a pivotal role in the regulation of body fat distribution. Adipogenesis is a process whereby multipotent adipose stem cells (ASCs) initially become preadipocytes (ASC commitment to preadipocytes) before differentiating into adipocytes. Androgens inhibit human (h) subcutaneous (SC) abdominal preadipocyte differentiation in both sexes, but their effects on hASC commitment to preadipocyte formation is unknown. We therefore examined whether androgen exposure to human (h) ASCs, isolated from SC abdominal adipose of nonobese women, impairs their commitment to preadipocyte formation and/or subsequent differentiation into adipocytes. For this, isolated hASCs from SC abdominal lipoaspirate were cultured in adipogenesis-inducing medium for 0.5-14 days in the presence of testosterone (T, 0-100 nM) or dihydrotestosterone (DHT, 0-50 nM). Adipogenesis was determined by immunofluorescence microscopy and by quantification of adipogenically relevant transcriptional factors, PPAR gamma, C/EBP alpha and C/EBP beta. We found that a 3-day exposure of hASCs to T (50 nM) or DHT (5 nM) in adipogenesis-inducing medium impaired lipid acquisition and decreased PPAR gamma, C/EBP alpha and C/EBP beta gene expression. The inhibitory effects of T and DHT at this early-stage of adipocyte differentiation, were partially and completely reversed by flutamide (F, 100 nM), respectively. The effect of androgens on hASC commitment to a preadipocyte phenotype was examined via activation of Bone Morphogenic Protein 4 (BMP4) signaling. T (50 nM) and DHT (5 nM) significantly inhibited the stimulatory effect of BMP4-induced ASC commitment to the preadipocyte phenotype, as regards PPAR gamma and C/EBP alpha gene expression. Our findings indicate that androgens, in part through androgen receptor action, impair BMP4-induced commitment of SC hASCs to preadipocytes and also reduce early-stage adipocyte differentiation, perhaps limiting adipocyte numbers and fat storage in SC abdominal adipose. (C) 2013 Elsevier Inc. All rights reserved.
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