Journal
STEM CELLS AND DEVELOPMENT
Volume 20, Issue 5, Pages 759-768Publisher
MARY ANN LIEBERT INC
DOI: 10.1089/scd.2010.0232
Keywords
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology
- Department of Transplantation for Regeneration Therapy (Sponsored by Otsuka Pharmaceutical Company, Ltd.)
- Molecular Medical Science Institute
- Otsuka Pharmaceutical Co., Ltd.
- Japan Immunoresearch Laboratories Co., Ltd (JIMRO), and Scientific research [18590388]
- Grants-in-Aid for Scientific Research [18590388] Funding Source: KAKEN
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The combination of allogeneic bone marrow transplantation (allo-BMT) and donor lymphocyte infusion (DLI) is a useful method for establishing donor chimerism and preventing a relapse of leukemia/lymphoma. However, there is a risk of inducing uncontrollable fatal graft-versus-host disease (GVHD). In fact, allo-BMT plus intravenous (IV)-DLI using donor splenocytes induces fatal GVHD in recipient mice. In this study, we examined the effects of the combination of intra-bone marrow (IBM)-BMT and the subcutaneous injection of donor splenocytes (SC-DLI) on the allo-BMT system. Recipient BALB/c mice were conditioned by sublethal irradiation (5 Gy), followed by IBM-BMT plus IV-DLI or SC-DLI in C57BL/6 mice. The IV-DLI group showed better engraftment of donor hemopoietic cells than the control group (without DLI) but showed fatal GVHD. The SC-DLI group, however, showed good reconstitution and mild GVHD. These results suggest that the combination of SC-DLI and IBM-BMT promotes the reconstitution of hemopoiesis and helps reduce the risk of GVHD.
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