Journal
STEM CELLS
Volume 29, Issue 6, Pages 981-991Publisher
WILEY
DOI: 10.1002/stem.646
Keywords
Strontium; Mesenchymal stem cells; Wnt/beta-Catenin
Categories
Funding
- National Natural Science Foundation of China (NSFC) [30970942, 30900594, 81000551]
- National Basic Research Program of China (973 program) [2009CB526501, 2010CB529605]
- Chinese Academy of Science
- Guangdong Innovation Research Team Fund for Low-cost Healthcare Technologies
- Shenzhen Governmental Basic Research Grant
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Strontium ranelate is a newly approved drug that can reduce the risk of vertebral fracture, which is attributed to its dual function in increasing the bone formation and decreasing the bone resorption. Strontium-containing hydroxyapatite was also demonstrated to stimulate the osteoblast activity and inhibit the osteoclast activity. However, the molecular mechanisms of strontium underlying such beneficial effects were still not fully understood. In this study, we investigated the effects of strontium on the osteogenic differentiation of human mesenchymal stem cells (MSCs) and its related mechanism; its osteogenic potential was also evaluated using a calvarial defect model in rats. We found that strontium could enhance the osteogenic differentiation of the MSCs, with upregulated extracellular matrix (ECM) gene expression and activated Wnt/beta-catenin pathway. After transplanting the collagen-strontium-substituted hydroxyapatite scaffold into the bone defect region, histology and computed tomography scanning revealed that in vivo bone formation was significantly enhanced; the quantity of mature and remodeled bone substantially increased and ECM accumulated. Interestingly, strontium induced an increase of beta-catenin expression in newly formed bone area. In this study, we showed for the first time that strontium could stimulate the beta-catenin expression in vitro and in vivo, which might contribute to the enhanced osteogenic differentiation of MSCs and in vivo bone formation. STEM CELLS 2011;29:981-991
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