Journal
STEM CELLS
Volume 26, Issue 10, Pages 2654-2663Publisher
WILEY-BLACKWELL
DOI: 10.1634/stemcells.2008-0285
Keywords
Rhesus monkey; Higher primates; Dental pulp stem/stromal cells; Bone marrow/mesenchymal stem/stromal cells; Cell therapy
Categories
Funding
- NIH [RR-00165]
- National Center for Research Resources/NIH [RR018827-04]
- NATIONAL CENTER FOR RESEARCH RESOURCES [R24RR018827, P51RR000165] Funding Source: NIH RePORTER
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Until now, interest in dental pulp stem/stromal cell (DPSC) research has centered on mineralization and tooth repair. Beginning a new paradigm in DPSC research, we grafted undifferentiated, untreated DPSCs into the hippocampus of immune-suppressed mice. The rhesus DPSC (rDPSC) line used was established from the dental pulp of rhesus macaques and found to be similar to human bone marrow/mesenchymal stem cells, which express Nanog, Rex-1, Oct-4, and various cell surface antigens, and have multipotent differentiation capability. Implantation of rDPSCs into the hippocampus of mice stimulated proliferation of endogenous neural cells and resulted in the recruitment of pre-existing Nestin(+) neural progenitor cells (NPCs) and beta-tubulin-III(+) mature neurons to the site of the graft. Additionally, many cells born during the first 7 days after implantation proliferated, forming NPCs and neurons, and, to a lesser extent, underwent astrogliosis, forming astrocytes and microglia, by 30 days after implantation. Although the DPSC graft itself was short term, it had long-term effects by promoting growth factor signaling. Implantation of DPSCs enhanced the expression of ciliary neurotrophic factor, vascular endothelial growth factor, and fibroblast growth factor for up to 30 days after implantation. In conclusion, grafting rDPSCs promotes proliferation, cell recruitment, and maturation of endogenous stem/progenitor cells by modulating the local microenvironment. Our results suggest that DPSCs have a valuable, unique therapeutic potential, specifically as a stimulator and modulator of the local repair response in the central nervous system. DPSCs would be a preferable cell source for therapy due to the possibility of a personalized stem cell, avoiding the problems associated with host immune rejection. STEM CELLS 2008; 26: 2654-2663
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