Journal
STARCH-STARKE
Volume 63, Issue 8, Pages 475-484Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/star.201000119
Keywords
Digestibility; Lima bean; Resistant starch; Structural characteristics; Velvet bean
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Velvet bean (Mucuna pruriens) and lima bean (Phaseolus lunatus) starches were isolated from seeds and their structural characteristics evaluated using XRD, size-exclusion chromatography and light scattering analyzes. Total starch, available starch, RS and in vitro digestibility were also determined. Structural and nutritional characteristics of Velvet bean and Lima bean starches were compared to those of commercial corn starch. The legumes starches presented a C-type XRD pattern and crystallite sizes of 43.1 angstrom for velvet bean and 48.3 angstrom for lima bean. Lima bean starch average molar mass (4.9 x 10(6) g/mol) was slightly higher than the velvet bean starch (3.04 x 10(6) g/mol). Size-exclusion chromatography indicated structural similarity between the lima bean and corn starches which differed from that of the velvet bean starch. Hydrodynamic radius (R-H) for the velvet bean and lima bean starches was 45.5 and 55 nm, respectively, and their radius of gyration (R-G) was 67.7 and 82.5 nm, respectively. Total starch content in all three starches was greater than 98%. Their complex crystalline structure provided the legume starches lower in vitro digestibility values than the corn starch. RS content in both the velvet bean starch (7.72%) and lima bean starch (5.66%) was higher than in the corn starch, essentially qualifying these polysaccharides as natural dietary fiber sources, with the associated physiologic advantages.
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