Journal
SPECTROSCOPY-AN INTERNATIONAL JOURNAL
Volume 22, Issue 5, Pages 327-343Publisher
HINDAWI LTD
DOI: 10.1155/2008/715905
Keywords
Metabolomics; human plasma; isotope labeling; quantitation; mass spectrometry
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Funding
- Swedish Research Council (VR)
- NIH [P30 MN062261]
- DOE [DE-AC02-05CH11231]
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Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.
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