Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 122, Issue -, Pages 22-33Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2013.11.006
Keywords
Copper(II)-dipeptide complex; 2-(2 '-pyridyl)benzimidazole; DNA binding; Oxidative cleavage; Antioxidant activity; HSA interaction
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Funding
- Program of Guangdong Provincial Science Technology [2011B020310005]
- South China Agricultural University [2009B010100001]
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A new ternary copper(II)-dipeptide complex [Cu(glygly)(HPB)(Cl)]center dot 2H(2)O (glygly = glycylglycine anion, HPB = 2-(2'-pyridyl)benzimidazole) has been synthesized and characterized. The DNA interaction of the complex was studied by spectroscopic methods, viscosity, and electrophoresis measurements. The antioxidant activity was also investigated using the pyrogallol autoxidation assay. Besides, the interaction of the complex with human serum albumin (HSA) in vitro was examined by multispectroscopic techniques. The complex partially intercalated to CT-DNA with a high binding constant (K-b = 7.28 x 10(5) M-1) and cleaved pBR322 DNA efficiently via an oxidative mechanism in the presence of V-c, with the HO center dot and O-2(-center dot). as the active species, and the SOD as a promoter. Furthermore, the complex shows a considerable SOD-like activity with the IC50 value of 3.8386 mu W. The complex exhibits desired binding affinity to HSA, in which hydrogen bond or vander Waals force played a major role. The alterations of HSA secondary structure induced by the complex were confirmed by UV-visible, CD, synchronous fluorescence and 3D fluorescence spectroscopy. (C) 2013 Elsevier B.V. All rights reserved.
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