Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 122, Issue -, Pages 153-157Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2013.11.015
Keywords
Spectrophotometric method; Bisphenol A; Diazotization-coupling reaction; Clenbuterol hydrochloride; HPLC
Categories
Funding
- key lab of analytical chemistry for Life science, Ministry of Education [KLACLS08009]
- Universities Natural Sciences Research Project of Jiangsu Province [12KJD610001]
- Science and Technology Program of Changzhou [CJ20120020]
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A new spectrophotometric method for the determination of trace amounts of bisphenol A based on a diazotization-coupling reaction was developed. In acidic solution, clenbuterol was first diazotized with sodium nitrite, then coupled with bisphenol A to from an azo-compound [I] in NH3-NH4Cl buffer, which shows a maximum absorption at 410 nm. The effects of the amount of sodium nitrite, diazo reaction time, the amount of clenbuterol, coupling reaction time and coupling reaction temperature have been examined. Under the optional conditions, the determination of the linear range of bisphenol A is 0.24-8.4 mu g/mL, correlation coefficient is 0.9905 and detection limit of this method is 0.15 mu g/mL. The spectrophotometric method is simple, rapid, high sensitivity with better accuracy. High performance liquid chromatography (HPLC) technique combined with this new spectrophotometric method has been also developed for the measurement of bisphenol A. The analysis was achieved on a C18 column using water and methanol as a mobile phase and the detection was done spectrophotometrically at 410 nm. These reported methods were applied to the determination of bisphenol A in hot water in contact with commercially available table-water bottle samples. (C) 2013 Elsevier B.V. All rights reserved.
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