Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 106, Issue -, Pages 224-230Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2012.12.097
Keywords
Ascorbic acid; Silver nanoclusters; Silver nanoparticles; Bovine serum albumin
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Funding
- National Natural Science Foundation of China (NSFC) [20965009, 21105087]
- National Innovation Experiment Program for University Students [101067322]
- Applied and Basic Research Program of Yunnan Province [2011FB014]
- Science Research Fund of Yunnan Province Education Department [2010Y242]
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We established a simple spectrophotometric and colorimetric method for detection of ascorbic acid based on the growth of silver nanoparticles in bovine serum albumin protected-silver nanoclusters (BSA-AgNCs) and Ag+ mixture. Due to the catalysis of BSA-AgNCs, ascorbic acid could reduce Ag+ to silver nanoparticles (NPs) at room temperature. The color of the mixture changed from colorless to yellow and a strong absorption band near 420 nm could be found in their absorption spectra owing to localized surface plasmon resonance (LSPR) of produced silver NPs. The absorbance changes at 420 nm had a good relationship with ascorbic acid concentration. Thus, we proposed a spectrophotometric and colorimetric method to determine ascorbic acid in concentration range from 2.0 to 50.0 mu M, with the corresponding limits of determination (3 sigma) of 0.16 mu M. (C) 2013 Elsevier B.V. All rights reserved.
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