Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 83, Issue 1, Pages 587-591Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2011.08.081
Keywords
Bioactive imidazole; FRET; Forester distance; Stern-Volmer plot
Categories
Funding
- Department of Science and Technology [SR/S1/IC-07/2007]
- University Grants commission [36-21/2008 (SR)]
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A novel Y-shaped imidazole derivative 4-((E)-2-(4,5-diphenyl-1-p-tolyl-1H-imidazol-2-yl)vinyl)phenol has been synthesized and characterised by IR, UV-vis, mass and NMR spectral techniques. The mutual interaction of this imidazole derivative (DPTIV) with bovine serum albumin (BSA) was investigated using photoluminescent studies. The fluorescence quenching mechanism of BSA by DPTIV was analyzed and the binding constant has been calculated. The binding distance between DPTIV and BSA was obtained based on the theory of Forester's non-radiation energy transfer. The effect of some common ions on the binding constant between DPTIV and BSA was also examined. (C) 2011 Elsevier B.V. All rights reserved.
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