Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 75, Issue 5, Pages 1435-1442Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2010.01.013
Keywords
Tryptophan; Erbium(III); Rare earth complexes; Herring sperm DNA; Interaction
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Funding
- Analytical and Testing Center of Southwest University of Science and Technology
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By means of UV and fluorescence spectra, the binding ratios between Er(III)-Trp and DNA in physiological pH environment (pH 7.40) were determined as n(Trp):n(Er)(III) = 3:1 and n(ER(III)(Trp)3) : n(DNA) = 2 : 1. and the apparent molar absorptivity of epsilon(Er(III)-Trp-DNA) is 4.33 x 10(5) L mol(-1) cm(-1) which was confirmed by molar ratio method. The binding constants at different temperatures K-B25 degrees C(theta) = 1.93 x 10(4) L mol(-1) and K-B37 degrees C(theta) = 5.28 x 10(3) L mol(-1) were obtained by double reciprocal method. Thermodynamic function computation demonstrates that Delta H-r(m)0 is the primary driving power of the interaction between Er(III)(Trp)(3) and DNA. By combination analysis of the Scatchard method and CD spectrometry, we suggested that the interaction mode between Er(III)(Trp)(3) complex and herring sperm DNA is groove and intercalation bindings. (C) 2010 Elsevier B.V. All rights reserved.
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