Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 77, Issue 2, Pages 466-472Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2010.06.020
Keywords
Bovine serum albumin; Benzodiazepines; Fluorescence quenching; Fluorescence resonance energy transfer; Thermodynamic parameters
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Funding
- Comision de Investigaciones Cientificas (CIC) de la Provincia de Buenos Aires, Argentina
- Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina
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The interactions of lorazepam, oxazepam and bromazepam with bovine serum albumin (BSA) were studied by fluorescence spectrometry. The Stern-Volmer quenching constants and corresponding thermodynamic parameters Delta H, Delta G and Delta S were calculated. The binding constants and the number of binding sites were also investigated. The distances between the donor (BSA) and the acceptors (benzodiazepines) were obtained according to fluorescence resonance energy transfer and conformational changes of BSA were observed from synchronous fluorescence spectra. (C) 2010 Elsevier B.V. All rights reserved.
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