β-Cyclodextrin cross-linked polymer as solid-phase extraction material coupled with the spectrophotometric method for the analysis of serum albumin

The beta-cyclodextrin cross-linked polymer (beta-CDCP) was synthesized and used as solid-phase extraction material to preconcentrate/analysis bovine serum albumin (BSA) coupled with UV-vis spectroscopy. The method based on the complex (BSA-phenylfluorone (PF)) adsorbed on beta-CDCP. Adsorption kinetics and various factors of the formation procedure of BSA-PF and its retention on beta-CDCP were investigated. The linear range and detection limit (DL) was 20.0-200.0 and 0.03 mg/L, respectively. Moreover. the beta-CDCP could be used repeatedly. The proposed method has been applied to analysis serum albumin with satisfactory results. (c) 2010 Elsevier B.V. All rights reserved.