Journal
SOUTH AFRICAN JOURNAL OF BOTANY
Volume 78, Issue -, Pages 44-46Publisher
ELSEVIER
DOI: 10.1016/j.sajb.2011.04.009
Keywords
Avocado fruit; cDNA; CTAB; Flesh; Polyphenols; Polysaccharides; Proteins; RNA extraction; Skin
Categories
Funding
- National Research Foundation, South Africa [ICD2007052500003]
- University of the Witwatersrand
- Bradlow Bursary
- Oppenheimer Memorial Trust
Ask authors/readers for more resources
The fruit of avocado is high in structural carbohydrates and proteins and also contains polyphenols which interfere with the isolation of high quantity and quality of RNA. The aim of this study was to optimize RNA isolation from avocado fruit for subsequent complementary DNA (cDNA) transcription. We describe an optimized cetyltrimethylammonium bromide (CTAB)-based RNA extraction protocol that allows effective extraction of high-quality total RNA from the skin and flesh of the fruit without the use of phenol. Total RNA obtained from this procedure is of high quality and is undegraded, as assessed by spectrophotometric readings and electrophoresis, and was successfully used for cDNA synthesis. This is a first report of effective RNA extraction from avocado fruit. (C) 2011 SAAB. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available