Journal
SOUTH AFRICAN JOURNAL OF BOTANY
Volume 76, Issue 1, Pages 25-29Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.sajb.2009.06.010
Keywords
Antibacterial activity; Antioxidant activity; D. viscosa; DPPH; Flavonoids; Free radical; MIC
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Funding
- The National Research Foundation
- Department of Labour-ScarceSkills Doctoral Scholarship
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Fractionation of dichloromethane and acetone fractions obtained by serial extraction from the leaf powder of Dodonaea viscosa Jacq. var. angustifolia (Sapindaceae) resulted in the isolation of four kaempferol methyl ethers. The compounds were identified by spectral data (H-1 NMR, C-13 NMR and MS) as: 3, 5, 7-trihydroxy-4'-methoxyflavone (1); 5, 7, 4'-trihydroxy-3, 6-dimethoxyflavone (2); 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin) (3); and 5-hydroxy -3, 7, 4'-trimethoxyflavone (4) together with 3,4',5,7-tetrahydroxy flavone (kaempferol) (5). Antioxidant potential of the compounds was evaluated using a DPPH spectrophotometric assay, while antibacterial activity was determined using a serial dilution microplate technique. The isolates demonstrated varying degrees of antioxidant and antibacterial activities. Of all the compounds investigated, compounds I and 5 demonstrated some antioxidant activity (EC50=75.49 +/- 1.76 mu M and 35.06 +/- 0.85 respectively) but lower than L-ascorbic acid (EC50=13.55 +/- 0.28 mu M) used as a standard antioxidant agent. The Minimum inhibitory concentration (MIC) of isolated compounds against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa varied from 16 mu g/ml to more than 250 mu g/ml. Some structure activity relationships could be established for these compounds. (C) 2009 SAAB. Published by Elsevier B.V. All rights reserved.
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