Journal
SOIL BIOLOGY & BIOCHEMISTRY
Volume 43, Issue 4, Pages 853-856Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2010.11.025
Keywords
Biological soil crust; Chlorophyll a; Dimethyl sulfoxide; DMSO; Ethanol; Methanol; Acetone
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Funding
- USDA Cooperative State Research Extension and Education Services (CSREES)
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We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chla from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chla recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chla using a two-extraction efficiency calculation. (C) 2010 Elsevier Ltd. All rights reserved.
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