Journal
ADVANCED HEALTHCARE MATERIALS
Volume 5, Issue 4, Pages 474-488Publisher
WILEY
DOI: 10.1002/adhm.201500520
Keywords
cardiac differentiation; conductive polymer; double-network hydrogels; stem cells; tissue engineering
Funding
- National Natural Science Funds for Distinguished Young Scholar [31125013]
- National High Technology Research and Development Program of China [SS2015AA020304]
- National Key Basic Research and Development Program of China [2011CB606206]
- International Cooperation and Exchanges in National Natural Science Foundation of China [31320103914]
- National Natural Science Foundation of China [31370975, 51573127, 31271016, 31100674]
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Cardiac tissue engineering is an effective method to treat the myocardial infarction. However, the lack of electrical conductivity of biomaterials limits their applications. In this work, a homogeneous electronically conductive double network (HEDN) hydrogel via one-step facile strategy is developed, consisting of a rigid/hydrophobic/conductive network of chemical crosslinked poly(thiophene-3-acetic acid) (PTAA) and a flexible/hydrophilic/biocompatible network of photo-crosslinking methacrylated aminated gelatin (MAAG). Results suggest that the swelling, mechanical, and conductive properties of HEDN hydrogel can be modulated via adjusting the ratio of PTAA network to MAAG network. HEDN hydrogel has Young's moduli ranging from 22.7 to 493.1 kPa, and its conductivity (approximate to 10(-4) S cm(-1)) falls in the range of reported conductivities for native myocardium tissue. To assess their biological activity, the brown adipose-derived stem cells (BADSCs) are seeded on the surface of HEDN hydrogel with or without electrical stimulation. Our data show that the HEDN hydrogel can support the survival and proliferation of BADSCs, and that it can improve the cardiac differentiation efficiency of BADSCs and upregulate the expression of connexin 43. Moreover, electrical stimulation can further improve this effect. Overall, it is concluded that the HEDN hydrogel may represent an ideal scaffold for cardiac tissue engineering.
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