4.7 Article

Biofabricated Nanoparticle Coating for Liver-Cell Targeting

Journal

ADVANCED HEALTHCARE MATERIALS
Volume 4, Issue 13, Pages 1972-1981

Publisher

WILEY
DOI: 10.1002/adhm.201500202

Keywords

-

Funding

  1. National Basic Research Program of China [2012CB933600]
  2. National Natural Science Foundation of China [51103043]
  3. 111 project [B14018]
  4. Innovation Program of Shanghai Municipal Education Commission [14ZZ060]
  5. Shanghai Science and Technology Development Funds [14QA1401000]
  6. Fundamental Research Funds for the Central Universities [WD1214056]
  7. United States National Science Foundation [CBET-1435957]

Ask authors/readers for more resources

Biology routinely uses noncovalent interactions to perform complex functions that range from the molecular recognition of ligand-receptor binding to the reversible self-assembly/disassembly of hierarchical nanostructures (e.g., virus particles). Potentially, biological materials that offer such recognition and reversible self-assembly functionality can be applied to nanomedicine. Here, polysaccharides with the multifunctional polysaccharide-binding protein Concanavalin A (Con A) are coupled to create a functional nanoparticle coating. This coating is self-assembled in a layer-by-layer format by sequentially contacting a nanoparticle with Con A and the polysaccharide glycogen. In the final assembly step, a galactomannan targeting ligand is self-assembled into the coating. Evidence indicates that the mannose residues of the galactomannan backbone are responsible for assembly into the coating by Con A binding, while the galactose side chain residues are responsible for targeting to the liver-specific asialoglycoprotein receptor (ASGP-R). Binding to ASGP-R induces endocytic uptake, while the low endosomal pH triggers disassembly of the coating and release of the nanoparticle-entrapped drug. In vitro cell studies indicate that the coating confers liver-cell-specific function for both nanoparticle uptake and drug delivery. These studies extend the use of Con A to sugar-mediated and organ-specific targeting, and further illustrate the potential of biologically based fabrication for generating functional materials.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available