Journal
ACS SYNTHETIC BIOLOGY
Volume 4, Issue 10, Pages 1144-1150Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.5b00054
Keywords
in vitro selection; strand displacement; riboswitch; aptamer; SELEX
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Funding
- Autonomous Province of Trento, Grandi Progetti, project Characterizing and improving brain mechanisms of attention-ATTEND
- Armenise-Harvard Foundation
- CIBIO
- National Science Foundation [61-2075UT]
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An in vitro selection method for ligand-responsive RNA sensors was developed that exploited strand displacement reactions. The RNA library was based on the thiamine pyrophosphate (TPP) riboswitch, and RNA sequences capable of hybridizing to a target duplex DNA in a TPP regulated manner were identified. After three rounds of selection, RNA molecules that mediated a strand exchange reaction upon TPP binding were enriched. The enriched sequences also showed riboswitch activity. Our results demonstrated that small-molecule-responsive nucleic acid sensors can be selected to control the activity of target nucleic acid circuitry.
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