Journal
SOFT MATTER
Volume 6, Issue 15, Pages 3499-3505Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c002265a
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Funding
- NUS Graduate School for Integrative Sciences and Engineering
- Global Enterprise for Micro-Mechanics and Molecular Medicine (GEM4)
- National University of Singapore [R279-000-214-133, R279-000-214-731]
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Image correlation spectroscopy (ICS) has been mostly used to quantify spatiotemporal events in biological experiments. Here, we develop and demonstrate the use of ICS for microrheology (mu R) of complex, viscoelastic materials. A special technique for extracting mean-squared displacements (MSD) of probe particles in the samples from image correlation data is developed. The method (ICS-mu R) is tested on Newtonian (glycerol solutions in water) and complex fluids (poly(ethylene oxide) (PEO) solutions). Accurate measurements of the viscosities of the Newtonian fluids (equivalently, the diffusion coefficients of the probe particles) as well as the storage and loss moduli of the PEO solutions could be obtained. The possibility to combine spatiotemporal assessment and time- and length-scale dependent microrheological measurements from images of fluorescent molecules makes ICS-mu R a prospective tool in many biophysical applications. Further, the method developed for extracting MSD from image (or intensity) correlation measurements can be applied to other microrheological techniques to provide improved accuracy of rheological measurements.
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