Journal
SOFT MATTER
Volume 5, Issue 11, Pages 2261-2268Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b903087e
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- NWO-ACTS
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We present a non-covalent immobilization system based on stimulus-responsive elastin-like polypeptides (ELPs) to facilitate the positioning of proteins in microchannels. Two ELP variants were constructed and connected to fluorescent proteins EGFP and DsRed2. These fusion proteins display an inverse transition behavior that can be simply controlled by varying concentrations of NaCl. With these ELP fusion proteins two patches of fluorescent proteins can be formed inside a microreactor, using only the temperature-responsive property of ELPs.
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