Journal
SMALL
Volume 14, Issue 40, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201802292
Keywords
gold nanoparticles; microRNA; signal amplification; upconversion nanoparticles
Categories
Funding
- National Natural Science Foundation of China [21335004, 21673108, 21605088]
- International Cooperation Foundation from Ministry of Science and Technology [2016YFE0130100]
- Natural Science Research Key Project of Education Department of Anhui Province [KJ2017A434]
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Intracellular microRNAs imaging based on upconversion nanoprobes has great potential in cancer diagnostics and treatments. However, the relatively low detection sensitivity limits their application. Herein, a lock-like DNA (LLD) generated by a hairpin DNA (H1) hybridizing with a bolt DNA (bDNA) sequence is designed, which is used to program upconversion nanoparticles (UCNPs, NaYF4@NaYF4:Yb, Er@NaYF4) and gold nanoparticles (AuNPs). The upconversion emission is quenched through luminescence resonance energy transfer (LRET). The multiple LLD can be repeatedly opened by one copy of target microRNA under the aid of fuel hairpin DNA strands (H2) to trigger disassembly of AuNPs from the UCNP, resulting in the lighting up of UCNPs with a high detection signal gain. This strategy is verified using microRNA-21 as model. The expression level of microRNA-21 in various cells lines can be sensitively measured in vitro, meanwhile cancer cells and normal cells can be easily and accurately distinguished by intracellular microRNA-21 imaging via the nanoprobes. The detection limit is about 1000 times lower than that of the previously reported upconversion nanoprobes without signal amplification. This is the first time a nonenzymatic signal amplification method has been combined with UCNPs for imaging intracellular microRNAs, which has great potential for cancer diagnosis.
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