Journal
SMALL
Volume 11, Issue 1, Pages 119-125Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201402016
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Funding
- Bundesministerium fur Bildung und Forschung (BMBF) [FKZ 0315316B]
- Bundesministerium fur Wirtschaft und Technologie (BMWi, Exist Research Transfer) [FKZ 03EFT9BW44]
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Efficient use of membrane protein nanopores in ionic single-molecule sensing requires technology for the reliable formation of suspended molecular membranes densely arrayed in formats that allow high-resolution electrical recording. Here, automated formation of bimolecular lipid layers is shown using a simple process where a poly(tetrafluoroethylene)-coated magnetic bar is remotely actuated to perform a turning motion, thereby spreading phospholipid in organic solvent on a nonpolar surface containing a <1 mm(2) 4 x 4 array of apertures with embedded microelectrodes (microelectrode cavity array). Parallel and high-resolution single-molecule detection by single nanopores is demonstrated on the resulting bilayer arrays, which are shown to form by a classical but very rapid self-assembly process. The technique provides a robust and scalable solution for the problem of reliable, automated formation of multiple independent lipid bilayers in a dense microarray format, while preserving the favorable electrical properties of the microelectrode cavity array.
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