Journal
SMALL
Volume 10, Issue 6, Pages 1212-1219Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201302161
Keywords
conjugated polymers; polymer nanoparticles; fluorescence imaging; cell tracing; morphology modulation
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Funding
- Singapore National Research Foundation [R279-000-390-281]
- Singapore Ministry of Defence [R279-000-340-232]
- SMART [R279-000-378-592]
- Economic Development Board (Singapore-Peking-Oxford Research Enterprise) [COY-15-EWI-RCFSA/N197-1]
- Institute of Materials Research and Engineering of Singapore [IMRE/11-1C0213, IMRE/13-8P1104]
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Single-chain conjugated polymer (CP) dots embedded nanoparticles (NPs) bearing cell penetration peptide (TAT) as surface ligands are synthesized for long term cancer cell tracing applications. The CPNPs are fabricated by matrix-encapsulation method and the embedded CPs can be modulated into spherical dots with different size upon alteration of feed concentrations. Single-chain CP dots are formed upon decreasing feed concentration to 0.2 mg/mL, where CPNPs exhibit highest fluorescence quantum yield of 32%. Maleimide is introduced as the new NP surface functional group, which favors easy conjugation with cell penetration peptide via click chemistry to preserve its biofunctions. The obtained CPNPs show high brightness and good biocompatibility, which allow cell tracing for over 9 generations, superior to commercial cell tracker Qtracker 585.
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