Journal
SMALL
Volume 9, Issue 13, Pages 2358-2367Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201202258
Keywords
nuleic acid delivery; lipoplexes; microwell arrays; theranostic medicine
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Funding
- National Science Foundation [EEC-0425625]
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Many delivery methods have been developed to improve the therapeutic efficacy and facilitate the clinical translation of nucleic acid-based therapeutics. A facile surface-mediated nucleic acid delivery by lipoplexes is prepared in a microwell array, which combines the advantages of lipoplexes as an efficient carrier system, surface-mediated delivery, and the control of surface topography. Uniform disc-like lipoplexes containing nucleic acids are formed in the microwell array with a diameter of approximate to 818 nm and thickness of approximate to 195 nm. The microwell array-mediated delivery of lipoplexes containing FAM-oligodeoxynucleotides is approximate to 18.6 and approximate to 10.6 times more efficient than the conventional transfection method in an adherent cell line (A549 non-small cell lung cancer cells) and a suspension cell line (KG-1a acute myelogenous leukemia cells), respectively. MicroRNA-29b is then used as a model nucleic acid to investigate the therapeutic efficacy of lipoplexes delivered by the microwell array. Compared to conventional transfection methods, the effective therapeutic dosage of microRNA-29b is reduced from the microgram level to the nanogram level by lipoplexes prepared in the microwell array. The microwell array is also a very flexible platform. Both nucleic acid therapeutics and imaging reagents are incorporated in lipoplexes and successfully delivered to A549 cells, demonstrating its potential applications in theranostic medicine.
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