Journal
SMALL
Volume 6, Issue 1, Pages 81-88Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.200901262
Keywords
fluorescence; imaging; molecular beacons; nanoparticles; RNA
Categories
Funding
- Nano Bio Regenomics Project
- 21st Century Frontier Research Program [2009K001257]
- MEST and NRF [R31-2008-000-10103-0]
- National Research Foundation of Korea [20090084640]
- Ministry for Health, Welfare and Family Affairs, Republic of Korea [A085136]
- National Research Foundation of Korea [2005-2000121, R31-2008-000-10103-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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An imaging system that can be used to evaluate the expression levels of microRNAs during neuronal development can provide noninvasive information for investigating a variety of biological phenomena related to microRNAs (miRNAs, miRs). Herein, the development of a novel imaging platform to monitor intracellular miR124a during neuronal differentiation is reported using rhodamine-coated cobalt ferrite magnetic fluorescent (MF) nanoparticles linked to a quenching molecular system containing an miR124a binding sequence (MF-miR124a beacon). During neuronal differentiation, in vitro fluorescence signals of the MF-miR124a beacon are significantly increased under conditions where miR124a is highly expressed, and dramatically return to the original quenched fluorescence after anti-miR124a treatment. In vivo fluorescence images show enhanced fluorescence signals in mice with P19 cells within a poly-L-lactic acid scaffold after induction of neuronal differentiation. In addition, magnetic resonance (MR) images provide in vivo tracking of cells containing the MF-miR124a beacon. These studies represent the first step toward the use of nanotechnological imaging of mature miRNA, and this technique could be used for cellular tracking with a MR imaging system as well as for simultaneous monitoring of the miRNA expression pattern in vivo.
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