Journal
SMALL
Volume 6, Issue 13, Pages 1415-1421Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.200902406
Keywords
biomolecules; continuous-flow; microarrays; microspotters; nanoporous materials
Categories
Funding
- Wasatch Microfluidics
- University of Utah
- State of Utah Centers of Excellence
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Biomolecules including protein A, albumin, and immunoglobulin C, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods. The spots are observed with a fluorescence scanner. By using the CFM to print spots on nanoporous substrates, an additional order of magnitude increase in signal is observed, which leads to high signal-to-background ratios, highly saturated spots, and a measurable signal at printing concentrations as low as 1.6 ng mL(-1). This technique produces highly concentrated biomolecular spots from dilute samples and significantly increases the sensitivity of sensing platforms.
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