Journal
THERANOSTICS
Volume 5, Issue 8, Pages 805-817Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.11520
Keywords
Photodynamic Therapy; Bioluminescence; Photosensitizers; Resonance Energy Transfer; Cancer; Photobiology; Photomedicine
Categories
Funding
- Korea National Research Foundation of Korea [R31-2008-000-10071-0]
- Bio & Medical Technology Development Program [2012M3A9C6049791]
- Mid-career Researcher Program of the National Research Foundation (NRF) - Korean government (MEST) [2012R1A2A2A06045773]
- MGH ECOR Fund for Medical Discovery
- US National Institutes of Health [U54CA143837, P41EB015903]
- NATIONAL CANCER INSTITUTE [U54CA143837] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [P41EB015903] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Optical energy can trigger a variety of photochemical processes useful for therapies. Owing to the shallow penetration of light in tissues, however, the clinical applications of light-activated therapies have been limited. Bioluminescence resonant energy transfer (BRET) may provide a new way of inducing photochemical activation. Here, we show that efficient bioluminescence energy-induced photodynamic therapy (PDT) of macroscopic tumors and metastases in deep tissue. For monolayer cell culture in vitro incubated with Chlorin e6, BRET energy of about 1 nJ per cell generated as strong cytotoxicity as red laser light irradiation at 2.2 mW/cm(2) for 180 s. Regional delivery of bioluminescence agents via draining lymphatic vessels killed tumor cells spread to the sentinel and secondary lymph nodes, reduced distant metastases in the lung and improved animal survival. Our results show the promising potential of novel bioluminescence-activated PDT.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available