Journal
SKIN PHARMACOLOGY AND PHYSIOLOGY
Volume 25, Issue 6, Pages 288-297Publisher
KARGER
DOI: 10.1159/000341222
Keywords
Antiseptics; Bacterial adaptation; Microplate laser nephelometry; Mupirocin; Staphylococcus aureus
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Funding
- Lohmann & Rauscher GmbH and Co. KG, Rengsdorf, Germany
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Background: Bacterial colonization and infection are important factors in compromised wound healing. Antiseptics have become an alternative for antimicrobial applications as antibiotic resistance is increasing; they have multiple targets with a broad spectrum of activity. Hence, the risk for developing resistance should be low. However, concerns have been raised that their growing use may result in bacteria that are less susceptible. Methods: The capacity of common antiseptics such as silver nitrate, polihexanide, octenidine, chlorhexidine and polyvinylpyrrolidone (PVP)-iodine to induce adaptation in a Staphylococcus aureus strain was analyzed in vitro using microplate laser nephelometry. S. aureus was repeatedly incubated with the respective half maximal inhibitory concentration (IC50) over a time period of 100 days. The influence of the continued treatment was determined by in situ monitoring of changes in the dose-response curves and calculation of the current IC50 values for the substances tested. Results: During the experiment, S. aureus quickly adapted to high concentrations of the antibiotic mupirocin during repeated treatment. Moreover, a significant increase of the IC50 for silver nitrate was observed over time. On the other hand, no significant difference was observed for polihexanide or chlorhexidine. While the IC50 for octenidine was also found to increase significantly, although the change was only marginal, reiterated incubation with PVP-iodine led to a decrease in the IC50. Conclusion: Repeated treatment of S. aureus with polihexanide, chlorhexidine, octenidine and PVP-iodine did not trigger bacterial adaptation to these substances. Copyright (C) 2012 S. Karger AG, Basel
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